A manual low-cost protein-crystallization plate jig for in situ diffraction in the home laboratory
نویسنده
چکیده
A prototype jig to attach a protein crystallization plate to a standard X-ray goniometer has been designed and constructed in partnership with an engineering firm. This allows a low-cost implementation of in situ diffraction using the available home-laboratory X-ray source.
منابع مشابه
Using high-throughput in situ plate screening to evaluate the effect of dehydration on protein crystals.
Crystal dehydration is a post-crystallization technique that can potentially improve the diffraction of macromolecular crystals. There are currently several ways of undertaking this process; however, dehydration experiments are often limited in their throughput and require prior manipulation of the samples. In the present study, a novel method is proposed that uses in situ plate screening to as...
متن کاملIn situ macromolecular crystallography using microbeams
Despite significant progress in high-throughput methods in macromolecular crystallography, the production of diffraction-quality crystals remains a major bottleneck. By recording diffraction in situ from crystals in their crystallization plates at room temperature, a number of problems associated with crystal handling and cryoprotection can be side-stepped. Using a dedicated goniometer installe...
متن کاملA simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids
A method is described for using plate lids to reduce evaporation in low-volume vapor-diffusion crystallization experiments. The plate lids contain apertures through which the protein and precipitants were added to different crystallization microplates (the reservoir was filled before fitting the lids). Plate lids were designed for each of these commonly used crystallization microplates. This sy...
متن کاملIn-plate protein crystallization, in situ ligand soaking and X-ray diffraction.
X-ray crystallography is now a recognized technique for ligand screening, especially for fragment-based drug design. However, protein crystal handling is still tedious and limits further automation. An alternative method for the solution of crystal structures of proteins in complex with small ligands is proposed. Crystallization drops are directly exposed to an X-ray beam after cocrystallizatio...
متن کاملs13.m37.o6 In situ pressure crystallization: an alternative to crystallization by cooling
In situ crystallization of liquids is usually connotated with the crystallization by lowering temperature, which started to be routinely applied in the 1950's [e.g. 1-4], and was perfected in the 1980's [5,6]. In situ temperature crystallization is routinely applied in neutron diffraction. In fact the low-temperature diffraction studies are regarded as the most accurate structural data. At the ...
متن کامل